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dpysl2 ko rats  (Cyagen Biosciences)
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Dpysl2 Ko Rats, supplied by Cyagen Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The information of the antibodies

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: The information of the antibodies

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques:

The information of the PCR primers and probes

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: The information of the PCR primers and probes

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques: Sequencing

The information of the DPYSL2/CRMP2 in the two-dimensional (2-DE) gelelectrophoresis. a – c The PMF of the DPYSL2. b – d The Ions score of protein spot of DPYSL2 by MASCOT software

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: The information of the DPYSL2/CRMP2 in the two-dimensional (2-DE) gelelectrophoresis. a – c The PMF of the DPYSL2. b – d The Ions score of protein spot of DPYSL2 by MASCOT software

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques: Software

The gene expression level (standardized CT) of the identified proteins in the four groups by Q-PCR. a The results of DPYSL2 and CRMP1 by Q-PCR in the four groups. b The results of other detected genes by Q-PCR in the four groups. Data were presented as means ± SD. * P < 0.05. Each group contained 6 samples

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: The gene expression level (standardized CT) of the identified proteins in the four groups by Q-PCR. a The results of DPYSL2 and CRMP1 by Q-PCR in the four groups. b The results of other detected genes by Q-PCR in the four groups. Data were presented as means ± SD. * P < 0.05. Each group contained 6 samples

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques: Expressing

Correlation analysis of the factors detected by GeneMANIA . Through searching for http://www.genemania.org/ , we found that DPYSL2 was encoded to a family of collapsin response mediator protein (CRMP). Moreover, it has some physical interaction with Tubb3 (tubulin, beta 3 class III) and Numb

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: Correlation analysis of the factors detected by GeneMANIA . Through searching for http://www.genemania.org/ , we found that DPYSL2 was encoded to a family of collapsin response mediator protein (CRMP). Moreover, it has some physical interaction with Tubb3 (tubulin, beta 3 class III) and Numb

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques:

Construction and identification of the ORF-DPYSL2 lentivirus. a Information of the EX-Rn10192-Lv201recombinant. b Enzyme digestion products of EX-Rn10192-Lv201recombinant, separated by 3% agarosegel electrophoresis. Lane 1, 4, and 5: DNA Ladder. Lane 2: EX-Rn10192-Lv201 plasmid. Lane 3: EX-Rn10192-Lv201 plasmid digested by NspVandXhoI. c Represent NSCs pictures taken by inversed fluorescent microscope before and at 3 and 7 days post transfection. Green: eGFP positive cells. Scale bar = 50 μm. Nor, normal group, neural basal medium addition; eGFP, eGFP group, eGFP-lentivirus transfection; DPYSL2, DPYSL2 group, DPYSL2-ORF vector transfection

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: Construction and identification of the ORF-DPYSL2 lentivirus. a Information of the EX-Rn10192-Lv201recombinant. b Enzyme digestion products of EX-Rn10192-Lv201recombinant, separated by 3% agarosegel electrophoresis. Lane 1, 4, and 5: DNA Ladder. Lane 2: EX-Rn10192-Lv201 plasmid. Lane 3: EX-Rn10192-Lv201 plasmid digested by NspVandXhoI. c Represent NSCs pictures taken by inversed fluorescent microscope before and at 3 and 7 days post transfection. Green: eGFP positive cells. Scale bar = 50 μm. Nor, normal group, neural basal medium addition; eGFP, eGFP group, eGFP-lentivirus transfection; DPYSL2, DPYSL2 group, DPYSL2-ORF vector transfection

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques: Electrophoresis, Plasmid Preparation, Microscopy, Transfection

The detection of NSCs differentiation toward neurons and astrocytes after DPYSL2-ORF transfection. a – c , e – g , i – k The immunofluorescent staining of DPYSL2 (red immunofluorescence), Tuj1 (green immunofluorescence), and GFAP (red immunofluorescence), respectively, in the three groups (Nor, eGFP, DPYSL2) at 7 days after transfection, DAPI stained the cell nucleus (blue). d , h , l The representative bar graphs for the ratio of the positive cells in the three groups. Data were presented as means ± SD. * P < 0.05. Scale bar, 25 μm. Each group contained 5 samples

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: The detection of NSCs differentiation toward neurons and astrocytes after DPYSL2-ORF transfection. a – c , e – g , i – k The immunofluorescent staining of DPYSL2 (red immunofluorescence), Tuj1 (green immunofluorescence), and GFAP (red immunofluorescence), respectively, in the three groups (Nor, eGFP, DPYSL2) at 7 days after transfection, DAPI stained the cell nucleus (blue). d , h , l The representative bar graphs for the ratio of the positive cells in the three groups. Data were presented as means ± SD. * P < 0.05. Scale bar, 25 μm. Each group contained 5 samples

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques: Transfection, Staining, Immunofluorescence

The impact of DPYSL2 knockout on the NSC differentiation into neurons. a – b DPYSL2-knockout rats plasmid construction and DPYSL2 expression identification. c Images of cultured hippocampal NSCs from WT (+/+) and KO (−/−) groups at 3 and 7 day as well as differentiation status. +/+, wild type rats; −/−, DPYSL2-knockout rats

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: The impact of DPYSL2 knockout on the NSC differentiation into neurons. a – b DPYSL2-knockout rats plasmid construction and DPYSL2 expression identification. c Images of cultured hippocampal NSCs from WT (+/+) and KO (−/−) groups at 3 and 7 day as well as differentiation status. +/+, wild type rats; −/−, DPYSL2-knockout rats

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques: Knock-Out, Plasmid Preparation, Expressing, Cell Culture

The impact of DPYSL2 knockout on the NSCs differentiation into neurons by immunofluorescent staining. The immunofluorescent staining of a , d , g nestin (red immunofluorescence), b , e , f Tuj1 (green immunofluorescence), and c , f , h GFAP (red immunofluorescence) in the +/+, DPYSL2 −/− Con group and DPYSL2 −/− PNS groups. DAPI was used to stain the cell nucleus (blue). Scale bar, 25 μm. i – k The representative bar graphs of the positive cells of nestin, Tuj1, and GFAP in these three groups. Data were presented as means ± SD. Each group contained 5 samples. * P < 0.05 versus DPYSL2 −/− Con group. Con, control group; PNS, Panax notoginseng saponins; +/+, wild type rats; −/−, DPYSL2-knockout rats

Journal: Stem Cell Research & Therapy

Article Title: DPYSL2 is a novel regulator for neural stem cell differentiation in rats: revealed by Panax notoginseng saponin administration

doi: 10.1186/s13287-020-01652-4

Figure Lengend Snippet: The impact of DPYSL2 knockout on the NSCs differentiation into neurons by immunofluorescent staining. The immunofluorescent staining of a , d , g nestin (red immunofluorescence), b , e , f Tuj1 (green immunofluorescence), and c , f , h GFAP (red immunofluorescence) in the +/+, DPYSL2 −/− Con group and DPYSL2 −/− PNS groups. DAPI was used to stain the cell nucleus (blue). Scale bar, 25 μm. i – k The representative bar graphs of the positive cells of nestin, Tuj1, and GFAP in these three groups. Data were presented as means ± SD. Each group contained 5 samples. * P < 0.05 versus DPYSL2 −/− Con group. Con, control group; PNS, Panax notoginseng saponins; +/+, wild type rats; −/−, DPYSL2-knockout rats

Article Snippet: CRISPR/CAS-mediated genome engineering was applied to construct DPYSL2-KO rats which were produced and provided by Cyagen (Guangzhou, China).

Techniques: Knock-Out, Staining, Immunofluorescence